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(6,7) The advent of 3D printed metal, ceramic, polymer, as well as composite scaffolds mimicking complex geometries and mechanical properties of the native tissue greatly expedited maxillary reconstruction in small animals. However, bone overgrowth of PTEB was a serious problem, requiring further modification of PTEB to shape it before performing a reparative surgery. (2) In these studies, a stable blood supply could be established in prefabricated tissue-engineered bones (PTEB) through endocultivation in a highly vascularized muscle pocket. Notably, PTEB based on TCP-BMP scaffold was successfully fabricated with pronounced effects on bone regeneration and vascularization based on radiographic, 18F-FDG PET/CT, and histological evaluation, suggesting a promising approach toward clinical translation.Ĭonventional cell-based tissue engineered constructs seem to be compromised in repairing large bone defects due to limited vascularization and lack of a mature vasculature network, which are crucial for providing adequate nutrient and oxygen supply to the adhered/migrated cells beyond 200 μm from the nearest blood capillary. Besides, there was a remarkable decrease in the PLGA/TCP-BMP scaffold density and increase in TCP-BMP scaffolds density during ectopic (within latissimus dorsi muscle) and orthotopic (within mandibular defect) implantation, indicating regular bone formation with TCP-BMP scaffolds. After 3-month’s prefabrication, the original 3D-architecture of the PLGA/TCP-BMP scaffold was found to be completely lost, while it was properly maintained in TCP-BMP scaffolds. 18F-FDG PET/CT was explored for real-time monitoring of bone regeneration and vascularization. Herein, 3D printed poly(lactic- co-glycolic acid)/β-tricalcium phosphate (PLGA/TCP) and TCP scaffolds with or without recombinant bone morphogenetic protein −2 (rhBMP-2) coating were utilized to repair primate’s large-volume mandibular defects and compared efficacy of prefabricated tissue-engineered bone (PTEB) over direct implantation (without prefabrication). However, studies related to their clinical translations are truly limited. More Pro AV + IS v5.1.889 Final + crack 9,490 วัน.The advent of three dimensionally (3D) printed customized bone grafts using different biomaterials has enabled repairs of complex bone defects in various in vivo models.
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